HuDSeN:258

SOX2 SRY (sex determining region Y)-box 2(NM_003106)
CS CS15
in situ hybridisation

Data Images
HuDSeN:258
Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes
The original images, scanned at high resolution, are available at http://nbb-slidepath.ncl.ac.uk username: hudsen password: See1Tuesday
Detection Reagent
Type:in situ hybridisation probe
Identifier:SOX2
Entity Detected:SOX2, SRY (sex determining region Y)-box 2(NM_003106)
Notes:697-bp fragment from the 3' untranslated region
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:human
Carnegie Stage:CS15
Preparation:section
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
hindbrain
strong strong
not applicable
dorsal root ganglion
moderate moderate
not applicable
eye
strong strong
not applicable
Spatial Annotation:
HuDSeN:258HuDSeN:258
3D mappingspatial mapping

Annotation colour key:   strong strong       moderate moderate     weak weak         possible possible     not detected not detected
Find spatially similar expression patterns: Find spatially similar patterns
Morphological match to the template: two stars
Spatial mapping approved by: EADHB Editor
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Principal investigator:Prof Mehul Dattani, Institute of Child Health, Clinical and Molecular Genetics Unit, 30 Guildford Street, London, UK WC1N 1EH
Submitted by:Janet Kerwin, Institute of Human Genetics, Newcastle University, International Centre for Life, Central Parkway, Newcastle upon Tyne, UK NE1 3BZ
Experiment type:non-screen
References:PMID: 18285410 Kelberman D, de Castro SC, Huang S, Crolla JA, Palmer R, Gregory JW, Taylor D, Cavallo L, Faienza MF, Fischetto R, Achermann JC, Martinez-Barbera JP, Rizzoti K, Lovell-Badge R, Robinson IC, Gerrelli D, Dattani MT 2008 SOX2 plays a critical role in the pituitary, forebrain, and eye during human embryonic development J Clin Endocrinol Metab (93):1865-73
Acknowledgments:The human embryonic material was provided by the Joint MRC-Wellcome Trust Human Developmental Biology Resource (http://www.hdbr.org) at the IHG, Newcastle-upon-Tyne, UK
Links: EMAGE same gene
  Allen Brain Atlas same gene
  BioGPS same gene

Entries are submitted to the HuDSeN database using the official gene name, according to the HUGO Gene Nomenclature Committee, at the time of uploading. If an official gene name subsequently changes after submission of the entry to the database, the previous name may be shown in the table above.


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