HuDSeN:658

PCNA proliferating cell nuclear antigen(J04718)
CS CS22
immunohistochemistry

Data Images
HuDSeN:658 HuDSeN:658
Embryo N300, slide 450, section 1. Embryo N300, slide 450, section 2.
Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Detection Reagent
Type:antibody
Identifier:PCNA antibody - Proliferation Marker
Entity Detected:PCNA, proliferating cell nuclear antigen(J04718)
Antigen:aa 1 - aa 200 of J04718
Notes:Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human PCNA.
Antibody Type:polyclonal
Raised In:rabbit
Supplier:Abcam
Catalogue Number:ab18197
Specimen
Organism:human
Carnegie Stage:CS22
Preparation:section
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
metencephalon
strong strong
not applicable
diencephalon
strong strong
not applicable
hypothalamus
strong strong
not applicable
cerebral cortex
strong strong
not applicable
future corpus striatum
strong strong
not applicable
Spatial Annotation:
HuDSeN:658HuDSeN:658HuDSeN:658
3D mapping3D mappingspatial mapping

Annotation colour key:   strong strong       moderate moderate     weak weak         possible possible     not detected not detected
Find spatially similar expression patterns: Find spatially similar patterns
Morphological match to the template: three stars
Spatial mapping approved by: EADHB Editor
Procedures
Specimen Fixation:4% paraformaldehyde
Embedding:paraffin
Labelled with:biotin
Visualisation method:DAB
General Information
Authors:Subrot Sarma
Principal investigator:Prof. Susan Lindsay, Institute of Genetic Medicine, Newcastle University, International Centre for Life, Central Parkway, Newcastle upon Tyne, United Kingdom NE1 3BZ
Submitted by:Janet Kerwin, Institute of Genetic Medicine, Newcastle University, International Centre for Life, Central Parkway, Newcastle upon Tyne, UK NE1 3BZ
Experiment type:non-screen
Links: EMAGE same gene
  Allen Brain Atlas same gene
  BioGPS same gene

The HuDSeN Gene Expression Spatial database currently contains data from CS12 to CS23. We are in the process of upgrading the database to include fetal gene expression patterns spatially mapped into 3D models. In the meantime the fetal gene expression images are available to view here.


Copyright ©The Medical Research Council & University of Newcastle
All Rights Reserved.